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Proteins resident in the inner nuclear membrane and underlying nuclear lamina form a network that regulates nuclear functions. This review highlights a prominent family of nuclear lamina proteins that carries the LAP2-emerin-MAN1-domain (LEM-D). LEM-D proteins share an ability to bind lamins and tether repressive chromatin at the nuclear periphery.


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View/Edit Mouse. LEM domain-containing protein 3 (LEMD3), also known as MAN1, is an integral protein in the inner nuclear membrane (INM) of the nuclear envelope. It is encoded by the LEMD3 gene [5] and was first identified after it was isolated from the serum of a patient with a collagen vascular disease. [6]


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The LEM (LAP2, emerin, MAN1) domain is a globular module of approximately 40 amino acids, which is mostly found in the nucleoplasmic portions of metazoan inner nuclear membrane proteins. The LEM domain has been shown to mediate binding to BAF (barrier-to-autointegration factor) and BAF-DNA complexes. BAF dimers bind to double-stranded DNA non.


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Analysis of the sequences of integral proteins of the inner nuclear membrane has identified a common structural domain of about 50 amino acids in three human and two Caenorhabditis elegans proteins [16].As this domain is present in LAP2, emerin, and MAN1, it has been termed LEM (Figure 1).All LAP2 isoforms share an N-terminal segment composed of such a LEM domain (residues 108-151) that is.


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Human LeMdomain gene and protein nomenclature Download Table

The nuclear lamina is an extensive protein network that contributes to nuclear structure and function. LEM domain (LAP2, emerin, MAN1 domain, LEM-D) proteins are components of the nuclear lamina, identified by a shared ∼45-amino-acid motif that binds Barrier-to-autointegration factor (BAF), a chromatin-interacting protein.Drosophila melanogaster has three nuclear lamina LEM-D proteins, named.


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The nuclear lamina is an extensive protein network that lies underneath the inner membrane of the nuclear envelope. This network establishes mechanical support for the nucleus and provides a platform for protein interactions that contribute to gene regulation, DNA replication and genome stability [1, 2, 3]. The major constituents of the nuclear.


Structure of the Drosophila LEM domain proteins. The Drosophila genome... Download Scientific

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Pancreatic cancer (PC) is a common type of malignancy originating from the epithelium of the pancreatic duct, with the most lethal feature and worst prognosis. LEM domain containing 1 (LEMD1) is overexpressed in multiple tumor tissues and plays a key role in cancer carcinogenesis and progression. Ho.


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Introduction. The nuclear lamina is an extensive protein network that lies underneath the inner membrane of the nuclear envelope. This network establishes mechanical support for the nucleus and provides a platform for protein interactions that contribute to gene regulation, DNA replication and genome stability [1-3].The major constituents of the nuclear lamina are the A- and B-type lamins.


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During the process of open mitosis in higher eukaryotic cells, the nuclear envelope (NE) is disassembled and reassembled with highly organized and periodical dynamic morphological changes. Recent studies demonstrated that LEM-domain protein family mediates interactions among inner nuclear membrane,.


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In mammals, three major types of LEM proteins can be distinguished based on their domain organization as shown in Fig. 1 (Brachner & Foisner, 2011).Emerin and LAP2 are INM proteins characterized by one transmembrane segment (Fig. 1 A).They have an N-terminal nucleoplasmic LEM domain (shown for LAP2 in Fig. 1 A) and a large region predicted as unstructured, followed by the transmembrane segment.


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Full article The nuclear envelope LEMdomain protein emerin

Emerin has roles in signaling, mechano-transduction, nuclear architecture, chromatin tethering and gene regulation. Also depicted are enzymes and pathways that directly target or regulate emerin. "L" indicates the LEM-domain. "L-prime" [L'] in Lap2β indicates the DNA-binding "LEM-like" domain. OGT, O -GlcNAc transferase.


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cl02649: LEM Superfamily. LEM (Lap2/Emerin/Man1) domain found in emerin, lamina-associated polypeptide 2 (LAP2), inner nuclear membrane protein Man1 and similar proteins. The family corresponds to a group of inner nuclear membrane proteins containing LEM domain. Emerin occurs in four phosphorylated forms and plays a role in cell cycle-dependent.