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The effect of mRNA and carrier reagent only on viability (a), CD80 expression (b), and TNF-α secretion (c) in monocytes transfected with low dose equivalent to 62.5 ng/well and high dose.
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Small interfering RNA (siRNA) is an effective and specific method for silencing genes. However, an efficient and non-toxic carrier is needed to deliver the siRNA into the target cells. Tumor necrosis factor α (TNF-α) plays a central role in the occurrence and progression of rheumatoid arthritis (RA). In this study, we pre-synthetized a degradable cationic polymer (PDAPEI) from 2,6.
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Storage. Human TNF-α Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
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Recombinant human TNF-alpha (Catalog # 10291-TA) has a molecular weight (MW) of 52.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation). Bioactivity View Larger. Recombinant Human TNF‑ alpha (HEK293-expressed.
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Bioactivity View Larger. Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED 50 for this effect is 25‑100 pg/mL. SDS-PAGE View Larger. 1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver.
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However, an efficient and non-toxic carrier is needed to deliver the siRNA into the target cells. Tumor necrosis factor α (TNF-α) plays a central role in the occurrence and progression of rheumatoid arthritis (RA). In this study, we pre-synthetized a degradable cationic polymer (PDAPEI) from 2,6-pyridinedicarboxaldehyde and low-molecular.
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Recombinant Human TNF-α (carrier-free) catalog : 570104. quantity : 50 μg. price : 235 USD. more info or order : BioLegend product webpage.
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TNF-alpha is a clinically important cytokine with strong pro-inflammatory activities. TNF-alpha is secreted by a variety of immune and non-immune cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. TNF-alpha signals through two receptors, TNFR1 and TNFR2. TNF-alpha has strong cytolytic activities against a wide variety of tumor cells and is.
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution.
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Recombinant Mouse TNF-α (carrier-free) Other Names : Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2. Size : 500 μg. Price (USD) : 1729 USD. Reactivity : Mouse.
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Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70ºC, avoiding multiple freeze-thaw cycles.. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked.
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In hepatitis B surface antigen (HBsAg) carriers undergoing anti-TNF therapy, either anti-HBV treatment or prophylaxis is mandatory to prevent hepatitis reactivation, whereas HBsAg-negative antibody to hepatitis B core antigen (anti-HBc) seropositive patients require watchful monitoring, only. Conversely, in HCV-infected patients, TNF-α.
