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Sheep were then challenged by subcutaneous injection of either BTV-16 (2 groups) or BTV-8 (5 groups). Taken together, 24/25 sheep from the 4 experimental groups developed detectable antibodies against the vaccinated viruses. Furthermore, sheep that received heterologous vaccines showed significantly reduced viraemia and clinical scores for BTV.
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The Bluetongue virus serotype 8 (BTV-8) strain, which emerged in Europe in 2006, had an unusually high ability to cause foetal infection in pregnant ruminants. Other serotypes of BTV had already been present in Europe for more than a decade, but transplacental transmission of these strains had never.
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A Seg-2 serotype-specific real-time RTPCR identified BTV-4, BTV-8, BTV-10 and BTV-16. This finding represents the first detection of BTV-8, BTV10 and BTV-16 in Brazil and, additionally, the first detection of BTV genomes in semen from bulls in the Country. RNA from multiple BTV-serotypes was detected in two individual semen samples suggesting.
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In Lebanon, BTV-1, -4, -6, -8, -16, and -24 were reported in 2011. BT has also been reported from Middle East countries like Iran, Iraq, Oman, Jordan, Saudi Arabia, Kuwait, etc (Maan et al. Citation 2011). A sporadic outbreak of BTV-10 was reported till 1998 in Europe (Wilson and Mellor Citation 2009).
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In this study, we developed and evaluated the beta-propiolactone inactivated bivalent bluetongue virus (BTV) serotypes 4 and 16 vaccine delivered with Montanide™ ISA-71VG adjuvant. The safety, stability and immunological profile of the fresh and after three years of long-term storage of the vaccine.
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Abstract. Since 1998, bluetongue virus (BTV) serotypes 1, 2, 4, 6, 8, 9, 11 and 16 have spread throughout Europe. In 2006, BTV serotype 8 (BTV-8) emerged unexpectedly in northern Europe throughout a region including Belgium, France, Germany, Luxembourg and the Netherlands. In the following year, it spread rapidly throughout the rest of Europe.
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Although BTV-2, BTV-4, BTV-6, BTV-10, and BTV-16 are considered endemic to Israel, clinical signs of disease are uncommon. We report clinical signs of infection in cattle in Israel caused by BTV-8 and BTV-24. We also report active circulation of 5 BTV serotypes (BTV-4, BVT-5, BTV-8, BTV-16, and BTV-24) during 2008-2009.
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ELISA Results on Naïve or BTV-1, 2, 8, 9 or 16 Infected or Vaccinated Animals. A vast majority of the 319 BTV-naïve ruminant sera had an OD of less than 0.1. Only three sera (1%) had an OD between 0.15 and 0.25 (Figure 2 A). All seven sera representing the seven serotypes of EHD were negative. Similar results were obtained in the presence of.
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Six BTV serotypes (BTV-1, BTV-2, BTV-4, BTV-8, BTV-9, and BTV-16) are present in those regions coming from three different epidemiological systems. The first one is located in the eastern part of the Mediterranean Sea and the serotypes involved (BTV-1, BTV-4, BTV-9, and BTV-16) seem to come from the Near, Middle, and Far East..
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The Bluetongue virus serotype 8 (BTV-8) strain, which emerged in Europe in 2006, had an unusually high ability to cause foetal infection in pregnant ruminants. Other serotypes of BTV had already been present in Europe for more than a decade, but transplacental transmission of these strains had never been demonstrated. To determine whether transplacental transmission is a unique feature of BTV.
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In 2006, BTV serotype 8 (BTV-8). 8, 12, and 16 post infection were processed for qRT-PCR. Two of the three pools (n = 5) were processed from C. sonorensis collected on days 2, 6, 10, 14, and 18 dpi. Some pools were reserved for potential individual analysis. For processing, each pool was manually homogenized with a sterile pestle in 250 µL.
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In turn, one sample from 2004 given positive for BTV-16 resulted positive also for BTV-4; three samples from 2002 given positive for serotypes -2 and -9 (C T values 27.1, 28.9 and 28.9) were identified by the BTV CodeSet only as BTV-9; one tissue sample given positive from 2015 for BTV-4 (C T 27.0 by qPCR NS3) was identified by the BTV CodeSet.
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About BTV PUR ®. Active immunisation of sheep to prevent viraemia and to reduce clinical signs caused by bluetongue virus serotypes 1, 2, 4 and/or 8 (combination of maximum 2 serotypes). Active immunisation of cattle to prevent viraemia caused by bluetongue virus serotypes 1, 2, 4 and/or 8, and to reduce clinical signs caused by bluetongue.
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BTV serotypes 1, 2, 4, 8, and 16 of Italian isolates were kindly provided by Dr. Savini (Istituto Zooprofilattico Sperimentale dell'Abruzzo et del Molise "G. Caporale", Italy). These viruses were propagated in Vero cells. To isolate single plaques from endpoint dilutions an overlay with 1% agarose was placed on an infected cell monolayer.
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In Lebanon, BTV-1, BTV-4, BTV-6, BTV-8, BTV-16 and BTV-24 were identified in 2011 (El Hage et al. 2013). In other west Asian countries, BT has been reported from Iraq, Iran, Jordan, Oman, Saudi Arabia, Syria, Turkey and Yemen; the newer serotype, BTV-26, has been isolated from Kuwait (Maan et al. 2011).
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However, since 1998, there have been separate and repeated incursions of bluetongue into Europe and 6 of the 24 different serotypes (BTV-1, -2, -4, -8, -9 and -16) have been introduced into.